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After publishing about a moth he’d only seen in collections, CU �鶹ӰԺ researcher Ryan St Laurent travels to Florida and spots the elusive—and previously thought extinct—Cicinnus albarenicolus

On the second of two nights he spent deep in central Florida forests last week—dripping sweat, shrouded in swarms of flying ants and June beetles, well into the 20 kilometers he’d eventually walk monitoring his four traps—Ryan St Laurent saw the thing he’d come, but didn’t really expect, to see.

To anyone who hadn’t spent a dozen years studying it, the sandy brown wisp might have looked like a fragment of autumn leaf or a shred of bark, but St Laurent immediately recognized Cicinnus albarenicolus. He’d just never seen the moth alive before, let alone in the wild.

In fact, until November, St Laurent thought this new species of Mimallonidae, or sack-bearer moth, might be extinct (DNA barcoding of moth specimens in collections had identified it as a new species). Before November, it hadn’t been seen in its extremely limited Florida habitat since the 1960s.

Ryan St Laurent, a CU �鶹ӰԺ assistant professor of ecology and evolutionary biology and CU Museum curator of entomology, traveled to Florida last week to try finding the elusive Cicinnus albarenicolus moth.

When news came that a collector had found one of the presumed-extinct moths in a sliver of white sand scrub in the Florida peninsula, St Laurent, a �鶹ӰԺ assistant professor of ecology and evolutionary biology and CU Museum curator of entomology, had just finished writing a describing the new C. albarenicolus, comparing it with other Mimallonidae species.

“I had written that it might be extinct, so I had to revise the paper and bring in some additional co-authors,” St Laurent says. Then he learned about an upcoming scheduled burn in one of the very few areas where C. albarenicolus conceivably could be found, so he booked a flight to Florida.

“I don’t think this is the only population in existence, and I don’t think it’s going to get burned up and go extinct,” St Laurent said several days before flying to Florida. “But I want to go out there and at least try to get a couple of tissue samples in the event we can’t find it again.”

Needles and haystacks don’t adequately encompass his aim; he was trying to find a small brown moth in a 450,000-acre forest.

‘These look really cool’

But how does a scientist first steer his scholarship to a little-known and barely studied family of moths, a member of which may or may not have been extinct? For St Laurent, the path began during undergrad at Cornell, where he studied entomology and worked with museum insect collections. The collections manager encouraged him to find something that nobody else was working on, “but there was a lot of competition in butterflies and moths—it’s a popular group as far as insects go,” he explains.��

“I remember going through the collection, asking, ‘What am I going to work on?’ when I came across this particular family (of moth). I was like, ‘Well, these look really cool,’ but when I went to try to curate them, I realized there were no resources, no books, no field guides, nothing.”

Perfect, he thought. If nobody was working on that family, he would. He wrote his undergraduate honors thesis then pursued his PhD in charting the phylogeny, or tree of life, of this small group of moths. “Once you have a tree of life, you can start talking about them and you can contextualize them as a member of bigger butterfly and moth groups,” he says.

It wasn’t until St Laurent got to the Smithsonian for his postdoc that he had a chance to order mitochondrial sequencing on one of the Mimallonidae specimens that he’d identified as different from its family members. That sequencing showed it was genetically different from anything else in its family, so when St Laurent came to CU �鶹ӰԺ, he continued the project of sequencing specimens from various collections.��

Most of the specimens were many decades old, compounding the challenges of genetic sequencing. St Laurent worked with a Canadian lab that specializes in barcode sequencing—a technique that focuses on short sequences of genes—sending them prepared samples for testing. In one instance, St Laurent sampled the leg of one of the few recent specimens, which he put on a sequencing plate and sent to Canada in January, looking for further evidence that this was, in fact, a new species of moth.

The genes didn’t lie: It was.

A moth flies out of the darkness

As if discovering a new species isn’t a big enough deal, discovering that it’s not extinct after all is enough to drive any researcher from the lab and straight into the Florida thickets.

Among the things that make Mimallonidae��interesting, St Laurent says, is they belong to a superfamily with ancient lineage—more than 100 million years old—99% of which live in Central and South America. Only a handful of species in the family occur in North America, but the ones that do are (mostly) quite common.

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Ryan St Laurent set up four insect traps with moth-attractant LED lights.

Except, of course, for C. albarenicolus—endemic to small patches of Florida Scrub, made rarer still by habitat loss. “Only 10% of Florida Scrub is left,” St Laurent said before leaving for Florida, “and the scrub that does still exist is super isolated. We don’t know if those little pockets can support this moth at all.”

Through some scientific sleuthing and mapping the locations where collection specimens had been found, St Laurent narrowed possible C.��albarenicolus��habitat to six sites in the Florida peninsula: eastern Ocala National Forest, Weeki Wachee north of Tampa, Cassia and Cassadaga northeast of Orlando, the Archbold Biological Station on the Lake Wales Ridge in Central Florida and coastal southeast Florida in Port Sewall. Each location has or had the rare Florida Scrub habitat—specifically white sand, open canopy scrub, which C.��albarenicolus seemed to favor.��

“This particular family of moths, there’s a reason nobody studies them,” St Laurent said before leaving for Florida. “They’re really hard to find and really hard to raise in captivity. I’ve done field work all over the Americas, and I’m lucky if I see one or two a night in Central or South America. I’m very used to not being able to find these things, which is why I do a lot of work in collections.”

Still, he had to try. He flew to Orlando and then drove to the township of Cassia. He had previously seen a specimen in the American Museum of Natural History in New York City that had been found near Cassia in 1964. “I knew about that specimen, I knew the scrub in that area because I went hiking there years ago in grad school and found caterpillars, but I didn’t rear them,” St Laurent says, so that’s where he started.

The first night, he set up four traps resembling tall, narrow tents with a specialized moth-attractive LED inside—the aim being to lure insects to the light. Other insects arrived in the thousands, but no C.��albarenicolus.

The second night, he set up at a spot in the nearby Seminole State Forest where the trees open to an expanse of sandy soil and scrubby plants. At 8:49 p.m., “I’m standing there and this kind of pinkish moth comes out of the darkness, and it was very recognizable. Nothing else really looks like that, moth-wise.”

After that first moth, two more came. St Laurent knew he was seeing females, which fly right after sunset, so he collected them and raced them to his colleagues at the University of Florida in Gainesville. Collecting live females means collecting eggs, with the attendant potential of rearing them in the lab. If his colleagues are able to rear them, he says, he will receive progenitors and offspring.

As for seeing a moth that he’d only previously seen as a collection specimen, “I was just like, ‘Wow, I was right! It is here!’ My suspicion is the moth is all over the place in Ocala, but it’s rare and diffuse there. It’s a much more concentrated site in Seminole, surrounded by hardwood hammocks and the St. Johns and Wekiva rivers, so you have a better chance of finding something there.”��

The site in the Ocala National Forest is scheduled for a controlled burn associated with Florida scrub jay management, “which is probably good in the overall grand scheme of things,” St Laurent says, “but since we don’t know what the moth eats or when it’s active or its annual lifecycle or habitat requirements, I don’t know if the burning regime is appropriate.

“(The moth is) part of Florida’s multimillion-year history, and Florida is the only place in the world where it occurs. It may not be some top-down species that’s controlling the habitat, but it’s still a very important representative of the one-sixth of its family that’s found in North America, and this one is the only species endemic to the U.S. in this family. It’s a part of Florida heritage and U.S. heritage, and we need to protect it.”

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